February 1st to 5th 2016
Olhão, Portugal
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Metabolic Enzyme Activities as Indicators for Physiological Conditions in Early Developmental Stages of Amphiprion ocellaris

Scientific Exhibition
Natural Resources
Wednesday, February 3, 2016 -
18:30 to 20:00

Paul, N. 1 Kunzmann, A. 2

1Leibniz Center for Tropical Marine Ecology (ZMT), Bremen
2Leibniz Center for Tropical Marine Ecology (ZMT)

Growing popularity of tropical marine aquaria creates a great demand for tropical reef organisms, resulting in increasing pressure on natural coral reefs. As most ornamental species are still caught in the wild with mainly destructive fishing methods, ornamental fishing causes population decline and habitat loss. Detailed knowledge about the basic metabolism in early life stages of tropical fish could optimize maintenance and captive breeding of ornamental fish. Enzymatic analyses of specific key enzymes for anaerobic and aerobic metabolic pathways reveal the organisms’ physiological conditions. In this study, activities of the metabolic key enzymes, citrate synthase (CS), pyruvate kinase (PK) and lactate dehydrogenase (LDH) of eggs, larvae and juveniles of false clownfish (Amphiprion ocellaris) were investigated to establish a set of baseline data for this economically important ornamental fish species. Within all developmental stages, the oxidative enzyme CS showed the lowest activities, whereas glycolytic enzymes, PK and LDH, were at least five times higher. With ongoing ontogenetic development, enzyme activities followed a bell-shaped curve, with highest activities for PK and LDH in 7-8 days old larvae (PK: 91.48 +/- 16.55 U g-1 WMg; LDH: 76.87 +/- 12.09 U g-1 WMg), and highest CS activities in 1-2 days old larvae (7.47 +/- 0.78 U g-1 WMg). However, one of the key results of our study is that parental influences play an important role for metabolic enzyme activities in early developmental stages of A. ocellaris. Differences in enzyme activities due to parental origin may be higher than those given by developmental stages. Further investigations on differences in enzyme activities in offspring of one breeding pair, but different clutches are needed. This will show whether metabolic key enzymes are suitable as general indicators for overall physical conditions of early life stages.
keywords: 
ornamental fish, citrate synthase (CS), pyruvate kinase (PK), lactate dehydrogenase (LDH), parental effects

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